The Human interferon-alpha 2B protein from Ansh Labs is an E. coli sourced protein that has been purified by proprietary chromatographic techniques. Lyophilized from a (1 mg/ml) solution in containing 2.3 mg Sodium phosphate dibasic and 0.55 mg sodium phosphate monobasic buffer. Greater than > 98% purity. Dimers and aggregates - Over 94% monomers. The specific activity as determined in a viral resistance assay using bovine kidney MDBK cells was found to be 2.6 x 10E8 IU/ mg.. Endotoxin - < 0.1 ng/ug. Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 0.924 as the extinction coefficient for a 0.1% (1 mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of IFN alpha 2b as a Reference Standard. Lyophilized Interferon although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution IFN alpha 2b should be stored at 4C between 2-7 days and for future use below -18C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. For Research Use Only. Reference the directional insert on the company's website for more updated specifications as they may have changed. Ansh Labs, located just south of Houston Texas, is an FDA registered manufacturer of diagnostic test kits, antibodies, antigens, and proteins. Our scientists are experts in immunoassay development and have developed some of the most challenging hormone assays helping to advance research and clinical diagnostics in reproductive function, growth disorders, metabolism, and more.
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