LC 10ml Syringe of Honey Mushroom Liquid Culture Syringe Made from properly sterilized Light Malt Extract solution, inoculated and prepared under our laminar flow hood and sealed in a small bag, with label and sterile needle, and alcohol prep pad.We make all of our LCs in small batches, and is tested to assure sterility. All our liquid culture syringes are guaranteed alive and pure, and replaced free of charge if they are contaminated About the Media & 10ml Liquid Culture Syringes All liquid culture syringes are made with the most care possible to produce the longest lasting and highest quality product. They come sealed in an air tight plastic bag, capped with a proper non-leaking syringe cap, and come with a separately packaged sterile needle. They come with at least 10ml of liquid culture, and 1-2mls of air. This is so you can shake and properly breakup the clumping mycelium to ensure that even the smallest drop of liquid is able to inoculate the substrate. The Culture MediaThe sugar solution is approximately 1.5% to increase lifespan of the syringes. The lifespan is often more than a year, though this depends on species & storage conditions. The solution is properly sterilized at 15 PSI for 30 minutes in a autoclave. As the sterilizer cools off, the jars seal and form a vacuum. This vacuum is not released until inoculation, and it is done so using a sterile 0.2 micron filter syringe under a laminar flow hood in a clean room. The liquid culture solution is inoculated, incubated, and then extracted under a laminar flow hood in a clean room. All nearby surfaces are wiped down with hydrogen peroxide while wearing gloves. We treat the air with an aerosol of propylene glycol before work to kill all air born bacteria in the clean room.
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